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KMID : 0380020140290010072
Korean Journal of Biotechnology and Bioengineering
2014 Volume.29 No. 1 p.72 ~ p.80
Isolation and Nitrogen Removal Characteristics of Heterotrophic Nitrification- Aerobic Denitrifying Bacteria, Stenotrophomonas sp. CW-4Y
Lee Eun-Young

Lee Chang-Won
Abstract
CW-4Y was identified as Stenotrophomonas sp. by morphological and physiological characteristics, and phylogenetic analysis of its 16S rDNA gene sequence. Nitrogen removal by CW-4Y was analyzed in relation to the ammonium concentration, presence of organic carbon, carbon source, and carbon-to-nitrogen ratio (C/N). Stenotrophomonas CW-4Y has heterotrophic nitrification and aerobic denitrification abilities. Stenotrophomonas CW-4Y utilized only glucose as carbon sources, and heterotrophic nitrification and aerobic denitrification were observed regardless of the type of nitrogen source. The maximum ammonium removal rate of CW-4Y was 80 mg-N¡¤L-1¡¤d-1 and its denitrification rate of 192 mg- N¡¤L-1¡¤d-1at NO3?-N (about 280 ppm) in shake culture experiments at a C/N ratio of about 15 was about 30 times higher than those of other bacteria with the same ability.
KEYWORD
Heterotrophic nitrification, Aerobic denitrification, Stenotrophomonas sp. Ammonia
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